Objective: To investigate In vitro anticancer activity of ethanolic extract of pericarp and seed parts of Momordica charantia on colon cancer cell line.

Materials and Methods: In vitro anticancer activity was carried out by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide assay. Different concentrations of the ethanolic extracts were tested on cell lines HT29.

Results and Discussion: IC 50 value were determined by measuring absorbance at 570nm. The IC50 value of seed and Pericarp was found to be 62.84 µg/ml and 143.75µg/ml respectively. The Momardica charantia showed moderate to potent anticancer activity. The above results showed Momardica charantia seed was better therapeutic activity than pericarp in the treatment of colon cancer. The Therapeutic activity may be due to the phytoconstituents present in the seed extract and further the seed have to be evaluated for GCMS studies.