Priyamvada, Preeti Mishra, Anita Sha and Animesh Kumar Mohapatra

The present investigation aimed to screen the phytochemicals in the methanolic and petroleum ether leaf extracts of Achyranthes aspera and evaluate their antidiabetic and antioxidant activities by using an in vitro model. Phytochemical screening of the extracts revealed the presence of alkaloids, tannins, saponins, flavonoids, terpenoids, phenols and steroids. The results of α–amylase and α–glucosidase enzymes inhibition activities were found in a dose-dependent manner. The strongest activity was showed by methanolic fraction (55.0±0.50% for α–amylase and 53.06±0.23% for α–glucosidase inhibition at 160 μg/ml) compared to petroleum ether extract (51.87±00% for α–amylase and 46.0±0.22% for α–glucosidase inhibition at 160 μg/ml). The plant extracts were also examined for its antioxidant activities by using DPPH scavenging method. The DPPH assay exhibited significant antioxidant activity of isolated phytochemical compounds’. The DPPH radical scavenging activity of the methanolic extract (68±0.44% at 250 μg/ml) was higher than that of petroleum ether extract (63.06±0.56% at 250 μg/ml). Thus, in conclusion this study can recommend this plant due to the presence of antioxidant components which have potential prospective for the control of diabetes and the related condition of oxidative stress. This knowledge will be helpful in exploring more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics.

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