Ayesha Siddika, Arifa Sultana, Lipy Khatun and SM Masud Parvez

Phyllanthus fraternus G.L. Webster belonging to the family of phyllanthaceae is commonly found in northern part of Bangladesh and has been extensive traditional use in folk medicine for the treatment of many diseased conditions. The aim of present study was to explore the quantitative phytochemical, antioxidant, cytotoxic, thrombolytic and antimicrobial properties as well as in vitro α-amylase inhibitory activity of methanolic extract of stem (MESP) and root (MERP)of Phyllanthus fraternus. MESP and MERP contained a good amount of phenolic, flavonoids, proanthocyanidins and flavonols compounds and showed as the strongest scavenging activity with an IC₅₀ value of 23.26µg/ml and 8.22µg/ml for DPPH and of 12.698µg/ml and 10.67µg/ml for ABTS respectively. From in vitro clot lysis method MERP (33.17%) exhibited more potential result than MESP (11.76%) as compared to standard drug Streptokinase (68.92%). Furthermore, MESP and MERP inhibited α-amylase enzyme in a dose dependent manner and brine shrimp mortality rate was increased with increasing concentration of extracts. In addition, positive result of antibacterial activity against both Gram-positive and Gram-negative bacteria was observed with highest zone of inhibition at 150mg/10µl concentration. In summary, these findings suggest that MESP and MERP possess a wide range of pharmacologically important phytochemicals which exhibited strong antioxidant activity

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