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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 10, Issue 6 (2021)

4-Hydroxyisoleucine from Fenugreek: Preparation of high pharmacological strength extract and assay method development

Author(s):

GHARAVI Kashani V, Bashiri Sadr Z and Haghighi Jirandeh T

Abstract:

Our product marketed under the trade name FenuBet® was first standardized according to its trigonelline content but latest investigations showed that 4-Hydroxyisoleucine is more implicated in the antidiabetic effect of Fenugreek seeds.
In this study two main topics were covered: optimization of factors leading to the preparation of an extract with high pharmacological strength using multi-stage counter-current extraction and development and validation of a new, simple, and rapid HPTLC-Scanner densitometry method for quantitative determination of 4-Hydroxyisoleucine.
Extracts with plant to solvent ratio of 1/2.26 and 4-Hydroxyisoleucine concentration up to 1.28 mg/ml were obtained. The extraction yield showed that more than 82.5% of 4-Hydroxyisoleucine was recovered.
The quantification method was found to give compact spots (Rf = 0.36). The minimum detectable amount was found to be 22.5 ng/spot, whereas the limit of quantitation was found to be 160 ng/spot. The linear regression analysis data for the calibration plots showed good linear relationship with 𝑟2 = 0.998±0.001 in the concentration range 22.5-160 ng/spot. %RSD for method repeatability was under 5.5% which is satisfactory.

Pages: 09-12  |  1 Views   Downloads

How to cite this article:
GHARAVI Kashani V, Bashiri Sadr Z and Haghighi Jirandeh T. 4-Hydroxyisoleucine from Fenugreek: Preparation of high pharmacological strength extract and assay method development. J Pharmacogn Phytochem 2021;10(6):09-12. DOI: 10.22271/phyto.2021.v10.i6a.14243