Vijaya Lakshmi T, Samiksha U, Nikitha V, Prashanthi U, Jyothi V and Praneetha P

Background: Diabetes mellitus is a clinical condition characterized by hyperglycaemia in which an elevated amount of glucose circulates in the blood plasma leading to diabetic compications. Alpha amylase and alpha glucosidase inhibitors are used to achieve greater control over hyperglycemia in type 2 diabetes mellitus. Pointed guard also known as Parwal (Trichosanthes dioica) is a vegetable consumed mostly by the people of all parts of India. In the present study the methanolic extract of Trichosanthes dioica was studied for in-vitro alpha (α)-amylase and alpha (α)-glucosidase inhibitory activities.

Materials and Methods: The methanolic extract of fruits of Trichosanthes dioica (TDME) was prepared by maceration. In alpha-amylase activity, alpha-amylase solution (0.5 mg/mL) and substrate, 1% starch was used, and absorbance was measured at 540nm. In Alpha-glucosidase activity, alpha-glucosidase (0.5 mg/mL) and substrate, 5 mM p-nitrophenyl-alpha-D-glucopyranoside was used; absorbance was recorded at 405 nm.

Results: Different concentrations of Trichosanthes dioica were assessed for alpha amylase and alpha-glucosidase inhibitory activities with an IC₅₀ value 8.220mg/ml and 5.819mg/ml extract respectively and were well comparable with the standard drug, acarbose. Conclusion: The extract, TDME, exhibited significant alpha-amylase and alpha-glucosidase inhibitory activities in dose dependent manner and was comparable to that of standard drug, acarbose.

Pages: 201-205  |  465 Views  252 Downloads