Tunde A Owolabi and Buniyamin A Ayinde

Previous studies on the stem bark of Musanga cecropioides showed its probable anti-cancer properties, to buttress this claims, we aimed to isolate the specific constituent(s) responsible for such activities using bioactivity guided isolation. Vacuum liquid chromatography and other chromatographic evaluations of the aqueous fraction led to the isolation of two compounds one of which was tested on AU565 breast cancer cell line. The isolated compounds were subjected to mass spectrometry on JEOL JMS 600H-1 on electron impact EI+ ionization mode and NMR (¹H NMR and ¹³C NMR) were carried out on Brucker Avance 600 MHz Spectrometer at frequencies of 400 and 800 MHz respectively in DMSO to determine their respective identities. One of the isolated compound (Catechin) and the aqueous fraction showed higher anti-cancer effects than the other fractions with 20.86 and 28% cytotoxicity on AU565 cancer cell lines at 50 µg/mL. The isolated compound was identified as Catechin. The results showed that Catechin is one of the anti-cancer constituents of M. cecropioides.

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