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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 10, Issue 6 (2021)

Antibiogram pattern of AMR E. coli from feed, water, boot socks and hand swab samples in pig farms located in and around Bengaluru, India

Author(s):

Shivakumar Rekhyanaik, Wilfred Ruban, Srikrishna Isloor, Ahlen IG Barry, Sharada Ramakrishna and Shivappa Nayaka

Abstract:

A study was carried out to evaluate the antimicrobial susceptibility pattern of E. coli isolated from feed, water, boot socks and human hand swabs of animal attender from 10 organized pig farms located in and around Bengaluru, Karnataka, India. All Samples from 10 organized pig farms (3 feed samples, 3 water samples, 3 Hand swabs and 2 samples of boot socks dust from inside the farm and one outside of the farm were collected from each farm) were collected and were used for isolation and characterization of E. coli based on biochemical methods. E. coli was isolated from all the samples. A total of 18 E. coli isolates obtained from feed samples, 12 E. coli isolates obtained from water samples, 25 E. coli isolates obtained from boot socks and 14 E. coli isolates obtained from hand swabs and were screened for antimicrobial susceptibility testing based on disc diffusion assay against 13 commonly used antimicrobials in human and veterinary medicine. Out of the of 18 E. coli isolates obtained from different feed samples revealed highest resistance to Doxycycline (94.44%) whereas the least resistance was observed in gentamicin, neomycin and cefpodoxime + clavulanic acid (5.56%), Among the 12 E. coli isolates from water samples 100 percent resistance was observed for Ciprofloxacin and the least resistance was observed in neomycin, chloramphenicol and amoxicillin each at 8.33 percent. Out of the 25 E. coli isolates recovered from Boot sock dust, 92 percent exhibited resistance towards doxycycline (Tetracyclines) and the least resistance was observed in amoxicillin (12%). Out of the 14 E. coli isolates recovered from animal attender hand swabs, 100 percent resistance was observed in Doxycycline and least resistance was observed in ceftazidime + clavulanic acid and Cefotaxime + clavulanic acid (14.29%) as well as amoxicillin (7.14%). However, none of the isolates recovered from water samples and animal attender hand swabs exhibited resistance towards gentamicin, amikacin, neomycin, imipenem and meropenem.

The phenotypic characterization confirmed the presence of Extended spectrum β-lactamase producing E. coli was highest in water samples (41.67%), followed by feed samples (27.78%), boot sock dust (20%) and the lowest ESBL presence was recorded in animal attender hand swabs (16.67%). The presence of Multidrug resistant (MDR) was highest in water samples (91.66%) followed by feed samples (88.88%), animal attender hand swab (85.71%), and boot sock dust (80%) being resistant to one antimicrobial agent in three or more antimicrobial classes. The study clearly indicated higher presence of multidrug resistant E. coli in feed, water, boot socks and human hand swabs of animal attender indicating its public health significance and potential of such samples being a major source in dissemination of AMR E. coli in the entire pork production chain.

Pages: 379-382  |  353 Views  126 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Shivakumar Rekhyanaik, Wilfred Ruban, Srikrishna Isloor, Ahlen IG Barry, Sharada Ramakrishna and Shivappa Nayaka. Antibiogram pattern of AMR E. coli from feed, water, boot socks and hand swab samples in pig farms located in and around Bengaluru, India. J Pharmacogn Phytochem 2021;10(6):379-382.

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