Hammylliende Talang, Abhijeeta Nandha, A Rekha, Hemanth, KV Ravishankar and Manjeet Talukdar
Jamun (Syzygium cumini Skeels.) is an important minor indigenous fruit of India. It belongs to the Myrtaceae family. Though native to India and Myanmar, it has naturalized throughout the South East Asia and Pacific regions. Jamun is an important fruit crop with a special character called polyembryony. This trait could be effectively utilized for propagation in nursery or hybridization work. The proper identification of zygotic and nucellar seedlings may lead to proper management of the elite germplasms in mass scale propagation. Conventional techniques of ‘Off type’ rouging and other morphological identification techniques are not full proof. Molecular marker may be used for discrimination of different seedlings. Cheap and cost effective RAPD technique is used in seedlings of a single seed of jamun with 20 non-specific primers. Primer OPP-04, OPT-06 and OPT-07 gave optimum polymorphism as compared to other primers. Total 11 amplicons were identified with these 3 primers that are effective in zygotic and nucellar discrimination. Sequencing of the amplicons could generate nucleotide sequence associated with the trait. Polyembryony character is regulated by several environmental factors, hence specific marker selection for specific Eco-region is crucial. The universal primers identified here are important for detection of polyembryony trait of jamun of this subcontinent.
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