Anoosha Thota, Varalaxmi Yellisetty and Maheswari Mandapaka
LEW1 a gene encoding a long-chain Cis-Prenyltransferase in dolichol biosynthesis. Dolichols play an important role in protein glycosylation process and unfolded protein response (UPR) pathway. In the present study LEW1 gene was isolated from Pennisetum glaucum. The LEW1 was cloned into pRT100 vector to mobilize the promoter and terminator. The cassette containing LEW1 gene was mobilized into plant expression vector pCAMBIA1300 with hygromycin phosphotransferase (hpt) as the selectable marker. The construct pCAMBIA1300-PgLEW1 was then mobilized into Agrobacterium tumifaciens strain LBA4404. Transgenic tobacco plants expressing PgLEW1 were generated and confirmed by PCR analysis using hpt and gene specific primers.
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