Rafeza Khatun, Farhana Khanam, Summaya Abedin Juhi, Naznin Ara Khatune and Md Aziz Abdur Rahman

The study was designated to perform antidiabetic and anti-inflammatory activities of the leaves of Lawsonia inermis L. Phytochemical screening of the plant was also evaluated. The crude ethanolic leaf extract (CEF 5.00 gm) was fractioned with pet-ether (PEF 2.65 gm) and ethyl acetate (EAF, 1.63gm). Anti-diabetic activity was assessed using alpha-amylase inhibitory assay with Acarbose as standard and found IC₅₀ (μg/ml) values of acarbose, PEF and EAF were 57.2, 206.11 and 323.1, respectively, indicating PEF as potent antidiabetic candidate compared to EAF. Anti-inflammatory activity was performed using hypotonic solution induced hemolysis and heat induced hemolysis. Ethanolic leave extract of PEF, EAF and standard aspirin at 800 µg/ml showed 80.0±0.79, 82.9±1.04 and 91.4±0.22% inhibition for heat induced haemolysis and 79.5±0.24, 84.8±0.05 and 96.3±0.16% inhibition for hypotonic solution induced haemolysis, respectively. The results showed EAF possessed strong anti-inflammatory activity compared to aspirin. The phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, resins, tannins, terpenoids and steroids and absence of anthocyanin, phlobatannins, proteins and saponin. From the observation it was concluded that Lawsonia inermis L might be a good source for anti-diabetes and anti-inflammatory agent.

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