Priyanka Sharma, Pawan K Verma, Shilpa Sood, Rasia Yousuf, NK Pankaj, Sanjay Agarwal and Anil K Pandey

The current study evaluated the potency of hydro-alcoholic Juglans regia hull extract against oxidative damage to testes in Wistar rats generated after isoprenaline (ISO) treatment. Thirty apparently healthy rats were divided into five groups with each group containing six animals. Various enzymatic and non-enzymatic parameters in testicular tissue like total antioxidant status (TAS), catalase (CAT), total thiols (TTH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), acetylcholinesterase (AChE), arylesterase (AE), malondialdehyde (MDA), advanced oxidation protein product (AOPP) were estimated. Impact of various treatments on sperm health related parameters and testicular histology was also evaluated. Subcutaneous administrations of ISO in rats caused significant rise in CAT, MDA, GPx, AOPP levels and fall of TTH, TAS, GR, AChE, AE levels in rat testicular tissue. Sperm abnormalities, declined sperm concentration along with histopathological changes in testicular tissue were also recorded in ISO treated rats. Pre-exposure with J. regia extract caused a significant reduction in ISO induced oxidative injury in testicular tissue as manifested by improvements in altered oxidative stress parameters and histopathological lesion scores. Observations of the present study revealed that extract prepared from hull of J. regia bestows a partial defense to testicles of rats against oxidative changes generated after treatment with ISO.

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