Himesh Soni and Jitender Malik

Flavonoids are universally in photosynthesizing  cells and are commonly found in various parts of nuts, fruit, vegetables, seeds, stems and flowers. Flavonoids are principal active constituents have been used to treatment of various human diseases. Rutin (quercetin-3- rhamnosyl glucoside) as the main flavonoid reported in leaves of Annona squamosa. It  is a multipurpose tree with eatable fruits & is a source one of the cosmetic products. Annona squamosa Linn is used in the treatment of diabetes, hepatotoxicity and in cancer cell line, It has been recognized that flavonoids display anticancer, antiviral, anti-inflammatory, and heart disease protective activities. The present study was carried out to isolation, characterization and antioxidant activity of rutin from leaves of Annona Squamosa from 80% ethanolic extract. The chemical compound isolated was analyzed by TLC, HPLC & IR. Several studies had been done for the isolation of rutin by different chromatographic method. In this study rutin was isolated from leaves of Annona squamosa by precipitation and fractional solubilization without the use of any chromatographic technique. The radical scavenging activities were carried out by various In-vitro and In-vivo methods. Rutin showed the most potent DPPH scavenging activity (IC₅₀ = 3.17 μg/ml) slightly higher than ascorbic acid (IC₅₀=4.92 μg/ml). The IC₅₀ value of lipid peroxidation assay for isolated rutin and BHT were 18.27 μg/ml and 46.79 μg/ml respectively.

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