YESSO Bogui Florian, TRÉBISSOU Jonhson Noel David and DJÉTOUAN Kacou Jules Marius

The active ingredients contained in the aqueous and hydroethanolic extracts of Adenia lobata was extracted used to conduct a toxicity study and evaluation of the anti-anemic activity of these extracts in anemic- induced rats. The dry powder of Adenia lobata was extracted with water (100g/L) for the aqueous extract and in a 70% hydroethanolic solution (ethanol/water 70:30) for the preparation of the hydroethanolic extract. The secondary metabolites contained in the different extracts were identified by thin layer chromatography. Acute oral toxicity was assessed according to the method of the Organization for Economic Cooperation and Development 423. Anemia was induced in rats by intraperitoneal administration of 40 mg/kg/d of phenylhydrazine for two days (J0 and J1) and these anemic rats were treated with extracts of Adenia lobata and vitamin B12. Adenia lobata extracts are rich in secondary metabolites such as flavonoids, alkaloids, polyterpenes but poor in quinone. The plant is non-toxic to the body because its LD₅₀ is greater than 5000 mg/Kg per body weight. The aqueous and hydroethanolic extracts of Adenia lobata normalize the hematological parameters of anemic rats. Adenia lobata could be a solution for cures of anemia encountered in children and pregnant women.

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