Le Van Nho, Pham Duc Thang, Vo Van Sy, Nguyen Huy Hoang and Pham Thu Huong

Pongamia pinnata (L.) Pierre grows in many places in Danang city, Vietnam. Local people normally boil fresh leaves of this species with hot water to obtain a solution used for showering to treat skin diseases effectively. Our study aimed to carry out the preliminary phytochemical screening of methanol extract and evaluate the antimicrobial activity of various fractions from Pongamia pinnata L. leaf, as well as to investigate the chemical compostion of the most effective fraction. Methanol extract and various fractions including n-hexan extract, chloroform extract, and ethyl acetate extract from leaves of Pongamia pinnata L. were prepared. The preliminary phytochemical screening of the extracts reveals the presence of alkaloids, flavonoids, organic acids, saponins and reducing sugar. Antibacterial activity of these extracts been studied by disc diffusion method against two bacterial strains, including Staphylococcus aureus ATCC 25923 and Methicillin-Resistant Staphylococcus aureus (MRSA). Among various extracts, the ethyl acetate extract exhibited the best antibacterial activity of. It can effectively inhibit the two strains of pathogenic bacteria, including S. aureus ATCC 25923 and MRSA with the sterile ring diameters of the test solution were 16.33 mm and 16.5mm, respectively. GC-MS analysis of ethyl acetate fraction led to the identification of nine compounds.

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