Twinkle J Bhatt, Dhruv Mehta, Vipul Nakum, Vivek Desai and Rakesh Sarvaiya

Arthritis is a chronic, inflammatory, systemic autoimmune disease, affecting the joints with varying severity among patients. The risk factors include age, gender, genetics, and environmental exposure. The objective of present study was to evaluate the anti-inflammatory activity of selected herbs by in-vitro protein denaturation. All the herbs present in the study are being used for the treatment of Arthritis since thousands of years and documented in many scientific studies. Selected herbs have different medicinal value and also having a nutritional value and due to that they will be fights against the arthritis by different mechanism. Herbal formulation shows the good therapeutic effect with minimum side effects as compared to the allopathic medication. The Hydro-alcoholic extracts in different concentration like 100 µg/ml, 200 µg/ml, 400 µg/ml, and 800 µg/ml of the selected herbs namely Adansonia digitata, Moringa oleifera, Rubia cordifolia, and Trapa natans incubated with bovine serum albumin for complete protein denaturation and the result was assess spectrophotometrically at 660nm against the standard diclofenac sodium. The percentage inhibition of protein denaturation for individual herb extract at 800 µg/ml showed higher activity so further the mixture of herb extract in equal proportion was prepared and evaluated at 800 µg/ml and it showed the more potent and significant anti-inflammatory activity. As the result of in-vitro study shows significant difference in protein denaturation, on the basis of that poly-herbal syrup was prepared and evaluated for its physiological parameters.

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