G Suresh and R Sutha Raja Kumar

The present investigation was carried out to identify the morphological and molecular characterization of the mushroom Tricholoma giganteum (TGS-1). The collected mushroom sample was identified as Macrocybe based on the morphological characters. Furrtherly, the Internal Transcribed Spacer (ITS) 1 and 2- PCR was employed to amplifying the rDNA from the pure culture of the mushroom and yielded 590 bp from the PCR. The amplified product was sequenced and identified using BLASTn in the NCBI. The BLASTn results revealed that, T. giganteum (TGS-1) strain was 98.82% similar with the strain Macrocybe giganteum (EU051917) and 98.65% with Tricholoma giganteum (MK024240). This mushroom was deposited as a new strain in NCBI GenBank with accession number MZ061712. The GC- MS analysis revealed that the presence of the biochemical compounds viz., 9,12,15-Octadecatrienoic acid, 2,3-dihydroxypropyl ester, (Z,Z,Z)-, 9,12-Octadecadienoic acid (Z,Z)-,2-hydroxy-1-(hydroxymethyl) ethyl ester, with highest peak area of 69.34% and high retention time (RT) of 22.81 min. This study revealed the novelty of the mushroom strain and encourage to further investigation on their benefits.

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