Tejashwini Dodamani and TC Taranath

Many studies have been suggested that nanotechnology effects on various science fields. This study was instantiates leaf broth for synthesis of silver nanoparticles. The various physicochemical parameters viz., pH, incubation period, temperature, quality and quantity of leaf extracts and, silver nitrate concentration were influences synthesis of the phyto engineered nanoparticles. Secondary metabolites were present in leaf extract play an important role in the green reduction of the silver ions involved in the capping of the AgNPs during synthesis this study revealed from the FTIR spectroscopic studies. pH 10 influences the formation of brown color and bioreduction of Ag+ to Ag ions observed in surface Plasmon resonance bands developed by UV-Vis spectroscopic studies data revealed that 419 nm. The negative charged particles study revealed by zeta potential. The XRD study reveals that crystalline nature of the nanoparticles and, smallest size of the nanoparticles at highly purity at 2θ 38⁰, lattice peak 111 and 15 nm. The peak was 1383 cm^-1 mainly involving the aromatic ring involved In the capping of the AgNPs during phytoreduction. Atomic force microscopic and HR-TEM image reveal that spherical shape and size of the NPs. The size of the nanoparticles is 10-80 nm in scale. Formation of nanofringes describes the high stability in nature. The efficacy of silver nanoparticles on antioxidant activity eas determined by the DPPH assay at different concentrations viz., 12.5, 25, 50, 100, 200 and 400 μg/ml. This investigation revealed that the significant decrease the colour intensity of the reaction mixture, when compared to the standard ascorbic acid. The determined IC₅₀ value of the silver nanoparticles was 50 μg/ml. These results were showed that a significant role in the increasing the radical scavenging activity.

DOI: 10.22271/phyto.2022.v11.i5a.14514

Pages: 50-54  |  477 Views  165 Downloads