Minoue Kuum Marc Germain, Tchadji Jules Colince, Mezui Christophe and Dimo Théophile
The present research was carried out to evaluate the effects of Albizia ferruginea aqueous stem bark extract (AFASBE) on the variation of some pro-inflammatory cytokines and haematological parameters during sub-chronic inflammation. Sub-chronic inflammation was induced in rats by the insertion of cotton pellets and then treated with AFASBE. Determination of the concentrations of TNF-α, IL1-β, IL-6 and INF-γ was carried out by the ELISA technique “Enzyme Linked Immuno Sorbent Assay” using the Quantikines kits (France). Haematological parameters were quantified using the Coulter S-Plus IV/D. The levels of TNF-α, IL-1β, IL-6 and INF-γ significantly (p<0.001) increased in the negative control serum compared to normal rats. AFASBE (100 and 200 mg/kg) and Diclofenac (4 mg/kg) induced significant (p<0.001) decrease of TNF-α, IL-1β, IL-6 and INF-γ levels compared to the negative control. Haematological analysis revealed that AFASBE (100 and 200 mg/kg) and diclofenac (4 mg/kg) significantly (p<0.05) reduced the number of white blood cells. The number of platelets decreased significantly (p<0.05), in all groups treated with AFASBE and diclofenac, compared to control. Albizia ferruginea aqueous stem bark extract is believed to have immunomodulatory potential on the inhibition of the expression of inflammatory mediators in serum during sub-chronic inflammation.
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