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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 12, Issue 1 (2023)

Formulating mixed variety of lotion prepared by standard Hypericum perforatum- Q and Calendula officinalis- Q with definite proportion with quality control by HPTLC and UV visible spectrophotometer

Author(s):

Dr. Suraj Singh Bhadoria, Dr. Monimala Pramanick, Dr. Mayank Roy, Dr. H Venkatesan, Dr. Manisha Prashant Gajendragadkar and Dr. Poorav Desai

Abstract:

Background: The Formulation of Prepared Mixed variety of Calendula officinalis lotion with Hypericum perforatum lotion as drug and Vehicle ratio is (1:1), which was prepared by Standard Calendula officinalis Mother tincture (Q) in distilled water (DW) and Standard Hypericum perforatum- Q in distilled water (DW) as drug and vehicle ratio is (1:9). 
Methodology: The formulation was prepared as drug and vehicle ratio (1:9). Where drug is taken as 1 part and vehicle is taken as 9 part. Thereafter Calendula officinalis- Q and Hypericum perforatum- Q is procure from GMP Certified pharmaceutical Pvt. Ltd., Which was taken as a 1 part in 9 ml of distilled water as a base line sample medium Seperately. Afterwards Mixed 1 part of Calendula officinalis lotion in 1 part of Hypericum perforatum lotion as (1:1) Quality assessment of mixed variety of Calendula officinalis and Hypericum perforatum lotion were done by the UV- visible spectrophotometer and HPTLC (High performance Thin layer Chromatography).
Result: While sample passing under UV- visible spectrophotometer, maximum absorption of Calendula officinalis- Q is 0.998 at 496 nm, Calendula officinalis lotion is 0.869 at 440 nm and Hypericum perforatum- Q is 0.994 at 642 nm, Hypericum perforatum lotion is 0.999 at 473 nm. On other hand, In HPTLC the Rf value under Calendula officinalis- Q is 0.744 with Value of X is 20 mm And Y is 61.6 mm and Rf value under Calendula officinalis lotion is 0.719 with X is 72.8 mm and Y is 59.8 mm, the Rf value under Standard Hypericum perforatum- Q is 0.804 with X is 46.4 mm and Y is 65.9 mm, Hypericum perforatum lotion is 0.761 with X is 99.2 mm and Y is 62.8 mm, whereas Rf value under Mixed Variety of Calendula officinalis and Hypericum perforatum Lotion (aqueous base) is 0.821 with X is 152 mm and Y is 67.1 mm.
Conclusion: Formulation prepared by mixed variety of Calendula officinalis- Q and Hypericum perforatum- Q gives a good result in quality control done by the UV- visible spectrophotometer and HPTLC (High performance Thin layer Chromatography).
 

Pages: 551-555  |  412 Views  157 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Dr. Suraj Singh Bhadoria, Dr. Monimala Pramanick, Dr. Mayank Roy, Dr. H Venkatesan, Dr. Manisha Prashant Gajendragadkar and Dr. Poorav Desai. Formulating mixed variety of lotion prepared by standard Hypericum perforatum- Q and Calendula officinalis- Q with definite proportion with quality control by HPTLC and UV visible spectrophotometer. J Pharmacogn Phytochem 2023;12(1):551-555.

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