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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 12, Issue 4 (2023)

In vitro, evaluation of anti-mycobacterial active plant extracts against Mycobacterium tuberculosis using BACTEC mycobacteria growth indicator tube 960 automated system

Author(s):

Vaibhav K Tamrakar, Jyothi Bhat, Nitish Singh Parihar, Hemant Thakur and S Rajasubramaniam

Abstract:

The emergence of drug-resistant Tuberculosis has become a global health problem. Herbal drugs promise other potential options for Tuberculosis treatment. This study aimed to investigate the anti-mycobacterial activity of three plants: Piper longum, Cressa cretica and Calotropis gigantea. The methanolic extract of these plants was tested against Mycobacterium tuberculosis H37Rv using the BACTEC MGIT-960 Automated system, and the minimum inhibitory concentration was determined. The extracts exhibited inhibitory activity against Mycobacterium tuberculosis H37Rv with Piper longum and Cressa cretica inhibiting at 125 μg/ml and Calotropis gigantea inhibiting at 250 μg/ml. However, the standard drugs isoniazid and rifampicin showed higher inhibitory activity against Mycobacterium tuberculosis at concentrations of 0.05 μg/mL and 0.12 μg/mL, respectively, compared to the crude plant extracts. This study highlights the anti-tuberculous potential of Piper longum and Cressa cretica as anti-mycobacterial agents. Nevertheless, further purification, molecular characterization and in vivo testing are necessary to evaluate their efficacy as novel anti-TB agents.

Pages: 63-65  |  678 Views  244 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Vaibhav K Tamrakar, Jyothi Bhat, Nitish Singh Parihar, Hemant Thakur and S Rajasubramaniam. In vitro, evaluation of anti-mycobacterial active plant extracts against Mycobacterium tuberculosis using BACTEC mycobacteria growth indicator tube 960 automated system. J Pharmacogn Phytochem 2023;12(4):63-65. DOI: 10.22271/phyto.2023.v12.i4a.14694

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