Samia Noor, Anika Tabassum Sheetol, Kazi Sanjida Alam Labonno, Tanjila Afroze and Anan Mahabub Aboni

The objective of this study was to investigate specific in vitro and in vivo properties of plant extracts derived from Rumex pulcher using the methanol extraction method. The primary method of assessment for the methanolic extract of Rumex pulcher (MERP) was established to examine the plant's in vitro capacity for various activities, including antioxidant, antimicrobial, cytotoxic, antinociceptive, and neuropharmacological activities. The reason for this phenomenon can be attributed to the pharmaceutical industry's keen interest in the various components of the plant. The DPPH Free Radical Scavenging Assay was employed to assess the antioxidant activity at various dosages. The disc diffusion method was utilized to assess the antimicrobial activity of the plant under investigation. Additionally, the Brine Shrimp Lethality assay was employed to determine the cytotoxicity of the plant. To perform in vivo antinociceptive testing, the hot plate method and acetic-acid-induced writhing tests were utilized. Involvement of opioid receptor was used for the confirmation of antinociception activity. The research conducted a neuropharmacological experiment employing the hole cross and open field methods. Acute toxicity was measured using Cinnamon oil induced method. The research findings indicate that MERP exhibits antioxidant activity with an IC50 value of 2.72 µg/mL. Additionally, the antimicrobial test revealed a dose-dependent zone of inhibition ranging from 08-18 µg/disc. The cytotoxic trial demonstrated a highly potent LC50 value of 1.28. The findings from the hot plate test and the acetic acid-induced writhing test demonstrated a significant antinociceptive effect, with a percentage of inhibition of 53.62% and 57.33% observed at doses of 200 and 400 mg/kg, respectively. Opioid receptor induced model confirmed the effectiveness of antinociceptive activity. The neuropharmacological investigation has demonstrated promising effectiveness. Acute toxicity test demonstrated mortality of the mice was increased after 2000mg/kg. In conclusion, the phytochemical found in this plant demonstrates robust pharmacological characteristics, rendering it a promising contender for drug exploration across diverse domains.

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