Mohd. Adil Tahseen, Aakash Bhatnagar and Ashish Bhatnagar

The search for novel, effective anti-diabetic medications with fewer side effects and less costs has switched to medicinal plants. The dried powder of leaves of Graptophyllum pictum was subjected to physicochemical evaluations i.e. moisture content was found to be 14. 56% and foreign matter content & swelling index were nil. The cold extractive values of leaves obtained from different media i.e. petroleum ether, chloroform, ethyl acetate, alcohol & water were found to be 0.44, 2, 2.9, 3.37 & 4.73% respectively. The obtained results showed that total, acid-insoluble, water-soluble and sulfated ash values were 1, 1.7, 18.2 & 19.1% respectively. The extractive values of leaves were found to be 1.9 & 7.5% for petroleum ether and ethanolic solvent respectively. TLC analysis of ethanolic extract was performed with different solvents i.e. CHCl3:CH3OH (5:1), CHCl3:CH3OH (5:2), C6H6:CHCl3:CH3OH (6:4:0.5) (v/v). The calculated Rf values ranged from 0.16-0.80. Chemical investigation of benzene fraction, obtained from ethanolic extract of leaves of G. pictum led to the isolation of two fatty acid esters namely n-octanyl capriate and n-decanyl cetoleate. GC-MS analysis of petroleum ether extract identified eight phytoconstituents namely Phytol, Tetracosane, Tetratertacontane, Tocopherol, Squalene, Vitamin E, Tetratriacontane and Stigmasterol. Administration of the extract at doses 250mg/kg and 500 mg/kg caused significant reduction in SGPT, SGOT, cholesterol and triglyceride levels. These investigations revealed that the extract possess potent antidiabetic activity.

DOI: 10.22271/phyto.2023.v12.i6c.14791

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