Devanshi Rajput, Payal Sargara and M Nataraj

Plants and their bioactive compounds are in medicinal practices since ancient times. Several medicinal plant species and their phytochemicals inhibit the progression and development of cancer. The present study aims to evaluate antioxidant potential, cytotoxic activity and apoptosis inducing ability of selected plant extracts prepared using different solvents for extraction. The extracts of Adansonia digitata (leaves), Punica granatum (peels) and Mangifera indica (peels) were prepared using three different solvents Methanol, Methanol: water (7:3) and Hexane: water (7:3). Their phytochemical screening revealed the presence of various secondary metabolites. The methanol: water extract of P. granatum (peels), A. digitata (leaves) and methanolic extract of M. indica (peels) confirmed robust antioxidant activity through DPPH radical scavenging method with an IC₅₀ value of 0.87µg/ml, 1.57µg/ml and 1.60µg/ml respectively. The methanolic extracts demonstrated substantial amount of cytotoxicity on Schizosaccharomyces pombe cells by affecting their growth and viability.The apoptosis inducing ability of plant extracts on yeast cells was checked through AO-EtBr dual staining. Further DNA isolation was carried out from yeast cells after 24h treatment of extracts showing high cytotoxicity and antioxidant activity. Results showed that control DNA was forming clear band while DNA of yeast cells treated with extract of A. digitata (leaves) (Methanol: Water), P. granatum (peels) (Methanol: Water) and M. indica (peels) (Methanol) were forming a smear indicated that extracts were altering the integrity of DNA. These plant extracts contain phytochemicals which are responsible for cytotoxic activity which may have potential to use as anticancer agents.

DOI: 10.22271/phyto.2024.v13.i1c.14826

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