Kanchan Kumari, Manoj Sharma, Pushpa Jagarwal and Mala Agarwal
Tinospora cordifolia and Glycyrrhiza glabra are well-known in traditional medical systems for its therapeutic effects, as it is efficacious in the management of infectious disorders while also reducing many of the adverse effects of synthetic antimicrobials. In an antibacterial bioassay, the biological activity of ethanol and benzene extracts of different plant components (leaves and stem) and callus of T. cordifolia and G. glabra was assessed. They could be used as lead compounds in the pharmacy sector or as the foundation for novel antibiotic research. Using the agar well diffusion method, the antibacterial activity of crude ethanol and benzene extracts of various plants parts (leaf and stem) and callus induced from nodal was assessed in vitro. The antimicrobial activity was determined using some S. aureus, B. subtilis, E. coli, S. grisveus, and fungal isolates viz. T. reesei, F. oxysporium, C. albicans and P. funiculosum. The ethanolic and benzene extract of callus showed maximum zone of inhibition in G. glabra and in T. cordifolia, leaf extract showed maximum toxicity against bacterial pathogens. Ethanolic and benzene extract of callus and stem was showed maximum zone of inhibition in T. cordifolia and G. glabra respectively against fungal pathogens. The leaves, stem and callus of T. cordifolia show that it had greater potential to inhibit the growth of all fungal pathogens but less toxic effect on C. albicans and F. oxysporum. The present study was carried out to estimate the antimicrobial activity of plants parts (leaf and stem) and callus extract to justify the pharmaceutical aspect of the plant to provide herbal plant products as phytochemistry in Ayurveda as well as ethno botanical aspect of the plant.
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