AY Saraf, AA Saraf, P Apurva and PS Shinde

Medicinal plants are reservoirs of therapeutic phytochemicals with potential to become a lead molecule for the development of novel drugs. ICH based HPTLC analysis is fast and simple method for developing chemical profile of herbals. The isolation and profiling of bioactive phytochemicals depends upon the extraction method. 
A comparative HPTLC fingerprint of hot and cold methanolic extracts of aromatic medicinal herbs viz. Rosemary (Rosmarinus officinalis L.), Oregano (Origanum vulgare) and Thyme (Thymus vulgaris L.) was developed on the CAMAG HPTLC system. The extracts were prepared by maceration, digestion, soxhlet and Ultrasound Assisted extraction methods. HPTLC studies were performed on 20 * 10 Silica gel 60 F 254 plates using Ethyl acetate: Formic Acid: GAA: Water (10:0.5:0.5:1.3 v/v/v/v) as solvent system to analyse the separation of phytochemicals under different extraction condition. 
Preliminary phytochemical analysis confirmed the presence of flavanoids in the extract. Quercetin was isolated and identified by using modified solvent system of Chloroform: Acetone: Formic Acid (76:16.5:8.5 v/v). The chromatogram was derivatized with ASR for isolating standard Quercetin. 
Among various extracts the soxhlet extract of Rosemary and Oregano showed better separation of phytochemicals. While all the four extracts of Thyme showed more or less similar separation of phytochemicals. Soxhlet is better method for separation of Quercetin in all the plants under study. Antioxidant activity of the extract was also evaluated by estimating DPPH radical scavenging assay. Oregano shows maximum antioxidant activity followed by Thyme and Rosemary.

DOI: 10.22271/phyto.2024.v13.i2f.14927

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