Naveen Saini, Preeti Goyal, Prashant Bakoria and Nishi Prakash Jain

The objective of the present study was to investigate and establish the antiamnesic/memory enhancing effect as well as the antioxidant capability of the ethanol extract of the leaves of Tabernaemontana divaricata. The extract was found to contain alkaloids, phenols, tannins, flavonoids and triterpenoids. The total phenolic content was determined and the total phenolics present in the ethanolic extract was found to be 44.86±2.689 GAE mg/g. The cognition enhancement potential of the ethanolic extract was determined by diazepam induced amesia model at two doses (200 mg/kg and 400 mg/kg) using actophotometer (locomotion) and pole climbing test (passive avoidance time). The percent improvement in locomotion compared to the disease control was found to be 24.49 and 29.31% respectively at the low and high dose of TLEE on the 8th day and 29.31 and 70.93% respectively at the low and high dose of TLEE on the 9th day suggesting a significant improvement in memory. On the other hand mice treated with TLEE were able to reduce the passive avoidance time by 13.29% at 200 mg/kg dose and 23.42% at 400 mg/kg dose on the 8th day whereas on the 9th day the time was further reduced by 27.75% at 200 mg/kg dose and 41.89% at 400 mg/kg dose on the 9^th day as compared to the disease control suggesting a significant improvement in memory. The antioxidant activity of TLEE was studied by assessing the total reducing potential by reduction of potassium ferricyanide (Fe³⁺) to form potassium ferrocyanide (Fe²⁺). The extract was able to produce time and dose-dependent reduction in the substrate suggesting antioxidant activity.

DOI: 10.22271/phyto.2024.v13.i3e.14978

Pages: 385-389  |  346 Views  135 Downloads