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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 13, Issue 5 (2024)

Spectrophotometric method for the determination of total alkaloids in selected plant parts

Author(s):

Digna Prajapati, Ami Makvana, Mohini Patel, Ranna Chaudhary and Ruby Patel

Abstract:

Total Alkaloids represent critical active constituents found within various plants. A swift, efficient, and straightforward spectrophotometric technique for quantifying total alkaloids, utilizing the reaction with bromocresol green (BCG), has been established for accurately determining total alkaloids in medicinal plants. At pH 4.7, a yellow complex is formed, which can be easily extracted using chloroform. The absorbance of this complex conforms to Beer’s law across a concentration spectrum of 4-13 µg atropine per ml of chloroform. This methodology can be executed in the presence of other compounds without any interference, thus underscoring the rationale behind the conducted study aimed at determining total alkaloids from twelve commonly found plants sourced from M.N. College, Visnagar. Extracts from the designated plant parts, including leaves, stems, seeds, and flowers, were prepared in acetone, and based on the subsequent investigation, it was conclusively determined that total alkaloids were present in Albizia lebbeck (L.) Benth., Crotalaria juncea L., Cucurbita maxima Duchesne, Dactyloctenium aegyptium (L.) Willd., (leaves, Echinochloa crus-galli (L.) P.Beauv., Eragrostis cilianensis (All.) Vignolo ex Janch., Hordeum vulgare L., Panicum miliaceum L., Senna occidentalis (L.) Link, Sesbania bispinosa (Jacq.) W. Wight and Setaria pallide-fusca (Schumach.) Stapf & C.E.Hubb., in which Crotalaria juncea L. Had the highest concentration (68.2 mg/ml), and lowest amount of alkaloid was present in Sesbania bispinosa (Jacq.) W.Wight (5.35 mg/ml).

Pages: 491-494  |  149 Views  58 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Digna Prajapati, Ami Makvana, Mohini Patel, Ranna Chaudhary and Ruby Patel. Spectrophotometric method for the determination of total alkaloids in selected plant parts. J Pharmacogn Phytochem 2024;13(5):491-494. DOI: 10.22271/phyto.2024.v13.i5g.15117

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