Dr. S Menaga, N Sudeepthi, C Chaadhuria, Dr. A Hannah Selvakumari and Dr. P Vijayashalini

Herbs are used in Therapeutic, Cosmetic and Food industry. The plants were extracted and then analyzed by using gas chromatography-mass spectrometry ^[1]. It is a sophisticated method that cannot be compared to other analytical tools available today, mass spectrometry can be used in conjunction with it to achieve GC-MS ^[2]. It is the perfect method for determining organic compounds that are volatile or semi-volatile both qualitatively and quantitatively in a wide range of samples ^[3]. Macroptilium atropurpureum was used as a forage crop, which is also used for their wound healing activity. To explore the bioactive compounds from the shade dried aerial parts of M. atropurpureum extracted with ethanol using Soxhlet apparatus and are subjected to GC-MS analysis. The rich bioactive compounds identified from the extract are lupeol and Phenol,2,4-bis(1,1- dimethylethyl)-,phosphite (3:1) which showed an eminent activity such as anti-inflammatory, anticancer, antioxidant and anti-enterococcal. These compounds hold significant commercial values find application in both pharmaceutical and indigenous medicine practitioners for the production of herbal or synthetic drugs ^[4]. The known beneficial outcomes were correlated with the type and concentration of the detected active compounds. Different amounts of the active principles with enormous amount of biological activity were found in the herb.

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