Munishama Gowda YN, Chaluvaraju KC and S Ramachandra Setty

This study aimed to develop and validate three analytical methods, including reversed-phase high-performance liquid chromatography, gas chromatography with flame ionization detection, and ultraviolet spectrophotometry, for the determination of eugenol isolated from the clove buds. The RP-HPLC technique utilized a C-18 column with a mobile phase composed of 0.1M orthophosphoric acid buffer solution and acetonitrile (70:30, v/v). For GC-FID analysis, DB-1 (USP Phase G2) column was employed for estimation. UV spectroscopy measurements were conducted between 200 and 800 nm using methanol as the solvent, with 282 nm chosen as the analytical wavelength. The developed analytical methods were validated according to the International Conference on Harmonization (ICH) guidelines Q2 (R1) for parameters such as linearity, accuracy, precision, specificity, and robustness. Consequently, each method provided highly dependable results for the quantification of eugenol isolated from Syzygium aromaticum L.

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