Joelri Michael Raj Lourdhurajan, Vasanth Nayagam, Anthony Samy and Mutheeswaran Subramanian

Plant extracts always provide ample opportunities for the discovery of newer bio-compounds for various applications. The specific orientation towards the isolation; purification and identification are the foundation for various functionalities addressed in modern sciences. Since plant residue possess numerous composite and promising cure elements, the present investigates the medicinal plant Tecomella undulata (sm.) Seem for various biological applications. The bioactive compounds isolated and crud drugs were analyzed for anti-bacterial activity and larvicidal activity. And the isolated compound by flash chromatography envisioned the possible zone of inhibition by specific compound isolated. For the evaluation of antibacterial study, three microbes viz., Vibrio sp., Staphylococcus albus and Proteus vulgaris were selected. Plant crude concentrations was (1.5, 2.5 and 5 mg/disc); and Streptomycin was the positive control. Notably out of these three extracts, the antibacterial effect of T undulate of the ethyl acetate extract was quite negative to certain pathogens. Proteus vulgaris showed 13 mm inhibition at 5 mg/disc concentration of the extract. Mosquito larvicidal effect of T. undulata extracts, 4^th instar larvae of Culex quinquefasciatus was used. Three extracts were taken at three different concentrations (62.5, 125, 250 ppm). The results were given as percentage of mortality (Table 4). For the estimation of larvicidal activity using hexane extract at 62.5 ppm concentration, R1, R2 and R3 showed 10% and the cumulative percentage was 10(%) out of 100%. R1, R3 morality was 10% and R2 was 20% morality and it resulted cumulative percentage 13.33(%). To evaluate the antibacterial activity of ethyl acetate extract five fraction has been taken by the flash chromatography - F1, F2, F3, F4, and F5. These five fractions were treated with same three pathogens that were used in the antibacterial effect of the extracts viz., Vibrio sp., Staphylococcus albus and Proteus vulgaris, and streptomycin was taken as the positive control. The cumulative results indicate the probable elements of T.undulata to ameliorate the biomedical and pharmaceutical sector to provide a positive underlying principle for assessing T.undulata for the treatment against bactericidal and mosquitocidal ailments.

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