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Journal of Pharmacognosy and Phytochemistry

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Journal of Pharmacognosy and Phytochemistry

Vol. 2, Issue 6 (2014)

Antimutagenic activity of root extract of Picrorhiza kurroa using Ames test in both dose dependant cytotoxic assay and mutagenicity study

Author(s): M. Hemanth kumar, C. Ramesh
Abstract: The study was performed to evaluate mutagenic induction potential of Picrorhiza Kurroa root extract. The extract was tested in the Salmonella typhimurium reverse mutation assay with five histidine-requiring strains of Salmonella typhimurium (TA1537, TA1535, TA98, TA100 and TA102). The test was performed in two independent experiments (viz., Dose Selection Study/Cytotoxicity Study and Mutagenicity Study) in the presence (10% S9 v/v) and absence of metabolic activation system in triplicates. In the dose range finding study, the extract was tested at the dose levels of 78.13, 156.25, 312.5, 625, 1250, 2500 and 5000 µg/ml in the presence (10% S9 v/v) and absence of metabolic activation system. The results of solubility, precipitation of test item and cytotoxicity study/dose range finding study, root extract of Picrorhiza kurroa was tested in the mutagenicity assay at the following dose levels. Mutagenicity Study with metabolic activation: 312.5, 625, 1250, 2500 and 5000 µg/ml. The root extract of Picrorhiza kurroa did not induce a significant dose-related increase in the number of revertant (his+) colonies in each of the five tester strains of Salmonella typhimurium (TA1537, TA1535, TA98, TA100 and TA102) in the presence (10% S9 v/v) and absence of metabolic activation system. Based on the results of this study it is concluded that root extract of Picrorhiza kurroa is not mutagenic in the Salmonella typhimurium reverse mutation assay (Ames test).Thus the study confirms the Antimutagenic effect of root extract of Picrorhiza kurroa.
Pages: 48-52  |  1404 Views  11 Downloads
How to cite this article:
M. Hemanth kumar, C. Ramesh. Antimutagenic activity of root extract of Picrorhiza kurroa using Ames test in both dose dependant cytotoxic assay and mutagenicity study. J Pharmacogn Phytochem 2014;2(6):48-52.
Journal of Pharmacognosy and Phytochemistry