Carrot seed essential oil, its fractions, isolated and derivatised compounds were screened for their nematicidal activity against plant parasite root knot nematode Meloidogyne incognita
. GC-MS analysis showed the presence of 51 compounds in carrot seed essential oil.
Sesquiterpenes are the main constituents of carrot seed essential oil out of which carotol, daucol and daucene were the major compounds whereas β-Cubenene and β- Farnesene were the minor compounds present. Carotol, daucol, daucene, polar and nonpolar fractions of carrot seed oil were isolated by column chromatography. Daucol and carotol were chemically derivatized. The isolated and derivatized compounds were characterized using FTIR, 1
H NMR and 13
C NMR spectroscopy. All these components viz
. carrot seed essential oil, its fractions, daucene, carotol, daucol, daucone, daucol acetate, 4-Chloro carotol and 3-Bromo carotol were evaluated for egg hatch inhibition and mortality of second stage juveniles (J2
) of Meloidogyne incognita
at different concentrations.
The nematicidal activity of carrot seed essential oil, carotol and polar fraction were found to be more effective than other components tested. Nematicidal activity was found to be concentration and time dependent.