To prepare agar plates (cultures) of Gram-positive Staphylococcus aureus (SA) and Gram- negative Pseudomonas aeruginosa (PA), Klebsiella pneumonia (KP) and Mycobacterium tuberculosis (MTB) individually.
Methods: Collection of plant, Microbial strain and culture preparation, Preparation of antimicrobial extract by solvent extraction, antimicrobial analysis, determination of inhibitory concentration, phytochemical analysis, purification of secondary metabolism.
Result: Antibacterial screening of Solanum S, Solanum nigrum and Datura stramonium: Anti bio gram analysis of Acetone, 80%Methanol, Chloroform and petroleum ether extracts of Solanum virginianum, Solanum nigrum and Datura stramonium extract against S. aureus (SA), Pseudomonas aeruginosa (PA), K. pneumoniae (KP) and Mycobacterium tuberculosis (MTB).
Conclusion: Project was done in the end of May throughout this project, we come up to a point where we can state that Solanum nigrum (makoi) and Datura stramonium (Datura) plants are good source of antimicrobial compound and can give to be a good source of natural medicine. They activity of antimicrobial can be enhanced by using metal ions, tested in different temperature and pH and can be tested in various other solvent. It has demonstrated to be powerful against bacteria and used at raise temperatures. So, we can terminate that drugs made out of would not be based on what conditions that are stored, this gives an edge in hold the drugs build out of this fruit.