Journal of Pharmacognosy and Phytochemistry
Vol. 9, Issue 5 (2020)
Diagnosis of Xanthomonas axonopodis pv citri isolates, the causal agent of citrus canker by pathogenicity and PCR based methods
Author(s):
Pradeep Manyam and Nargund VB
Abstract:
Citrus canker caused by
Xanthomonas axonopodis pv
citri (
Xac) is one of most destructive diseases of citrus in terms of damage to trees and fruit quality and remained as one of the important quarantine disease. Under field conditions, though Asiatic citrus canker disease is relatively easy to diagnose, the accurate detection of pathogen for various pathological studies is necessary. In present study, a total of 13 plant samples collected from various parts of Karnataka state were diagnosed by an integrated approach using pathogenecity, isolation and molecular techniques. Five isolates were able grow on nutrient glucose agar medium characteristic of
Xanthomonas axonopodis pv.
citri.. The pathogenicity of all the above five isolates (
XAC 1, 2, 3, 4 and 5) was proved with typical canker lesions on leaves of citrus after five to eight days of incubation period. All five isolates of
Xac were confirmed at molecular level by amplifying highly conserved nucleotide sequence
est A region (777 bp) using
Xc-lip primer pair that distinguishes Xanthomonads from bacteria. The study revealed that integrated approach as reliable technique for detection of
Xac for laboratory analysis.
Pages: 173-176 | 1283 Views 597 Downloads
Pradeep Manyam and Nargund VB. Diagnosis of Xanthomonas axonopodis pv citri isolates, the causal agent of citrus canker by pathogenicity and PCR based methods. J Pharmacogn Phytochem 2020;9(5):173-176.