Author(s):

Pranav Chauhan, Rajiv Ranjan Kumar, Sanjod Kumar Mendiratta, I Prince Devadason, Dhananjay Kumar, Mukesh Gangwar, Deepali T Sakunde and Somesh Kumar Meshram

Abstract: Vinca rosea a commonly growing ornamental plant of garden. Water and ethanolic extracts of leaves from Vinca rosea were prepared and evaluated for porcine pancreatic lipase inhibitory effect using DNPB (2,4-dinitrophenyl butyrate) and Triolein as substrate. The total phenolic contents of extracts, saponin content of leaves, DPPH radical scavenging activity and Ferric reducing power assays of both types of extracts were also measured. The lipase inhibitory activities of water and ethanolic extract of Vinca rosea leaves were 32.39% and 40.81% respectively using DNPB (2,4-dinitrophenyl butyrate as substrate. Using Triolein as substrate, values were 28.55% and 40.78% respectively. The total phenolic, saponin (%), DPPH free radical scavenging activity (%) and ferric reducing power assay (absorbance) were measured as 11-12 mgGAE/g, 1.34-1.81, 32.24-41.48 and 0.69-0.71 respectively. Both the extracts, water and ethanolic were same except the difference in pancreatic lipase inhibitory activity and DPPH radical scavenging activities which were higher in ethanolic. The study suggested that both water and ethanol extracts are capable as anti pancreatic lipase and antioxidant with slight edge for more polar ethanol and can act as promising natural alternates of the established lipase inhibitory molecules i.e. orlistat and antioxidant BHT.

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