Author(s):

Shiva kumar Chinchure and MB Patil

Abstract: Grain discoloration disease was first recorded as minor disease of rice in Karnataka, with yield losses approaching >30% under appropriate environmental conditions. Curvularia lunata is plant pathogenic fungus which is one of the dominant causal organism of grain discoloration of rice. The present work was done to assess the molecular variability in twenty isolates of Curvularia lunata, collected from five different agro-ecological regions of Karnataka, ranging from North and South irrigated maidan area, Southern transitional area, Hilly and coastal areas of Karnataka using random amplified polymorphic DNA (RAPD) markers. The similarity values of RAPD profiles ranged from 0.39 to 0.66 among all the isolates. Maximum similarity of 66% was observed between isolates Cl-14 and Cl-3 followed by 65 per cent between isolates Cl-8 and Cl-3. Out of twenty isolates 17 isolates were in cluster A, under various sub clusters showing some degree of variability. Most of the isolates collected from similar agro-ecological location clustered together in the present study

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