Author(s):

Grishma Lamichhane

Abstract: An experiment was conducted in the glasshouse of Soil Science Division under Nepal Agriculture Research Council (NARC), Khumaltar, Lalitpur from 16^th July to 6^th October, 2017 with the major objective to study the release of phosphates and change in pH of soil by Phosphate Solubilizing Microorganisms (PSM) in different types of soil. The experiment followed the principle of Completely Randomized Design with five treatments and three replications. The five treatments of the experiment were T₁ (Pine forest Soil), T₂ (Red Soil), T₃ (Swampy area Soil), T₄ (Clay Soil) and T₅ (Normal Cultivated Soil). 0.1% PSM was applied in each replication @ 5ml per seed with two seeds of French bean in each replication. Various parameters were observed and analysed using SPSS. Results of PSM application on different treatments revealed that the release of phosphates varied significantly among the treatments in different time interval. The highest phosphate release (390.57kg P₂O₅ ha^-1) was found in the treatment T₄ (Clay Soil) and the time of maximum release was recorded at 20 days after PSM application. Similarly, the change in pH after the PSM application was also found statistically significant between the treatments in different time interval. The maximum change in soil pH was recorded in treatment T₃ (Swampy Soil). Significant positive correlation was found between plant height and available phosphorus content of soil. Likewise, positive correlation was found between plant height and soil pH and between available phosphorus and soil pH but it was not significant. Concluding, application of PSM in soil can solubilize the insoluble phosphorus and make it available for plant uptake.

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