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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Special Issue 1 (2018)

Partial purification and characterization of hide dehairing protease

Author(s):

Sanjay Kumar Yadav, Poonam Singh, Kashyap Kumar Dubey and Bhanu P Singh

Abstract:
Partial purification of crude enzyme was carried out by ammonium sulphate precipitation, dialysis and ion exchange chromatography. Alkaline protease was purified upto 2.19 fold as compared with crude enzyme. The apparent molecular weight of partially purified enzyme was determined as 48 kDa by SDS-PAGE and the same was confirmed from zymography. The maximum alkaline protease production was achieved with 7% inoculums size at pH 10, 36 ºC incubation temperature, with impellor tip velocity of 4714 cm/min, and 48hrs of incubation period. Alkaline protease activity was completely inhibited by PMSF; while EDTA showed no inhibition on protease activity that is suggested that the enzyme is a serine protease. The Km and Vmax for the partially purified alkaline protease were 4.343mg and 1mg/min-1ml-1 respectively. Alkaline protease was used for hide deharing of goat and found that complete deharing was achieved with protease unit 200 U/ml and in 6 hrs having Mn2+ concentration of 10 mM with 50 0C temperature and 10pH

Pages: 2608-2611  |  1070 Views  228 Downloads

How to cite this article:
Sanjay Kumar Yadav, Poonam Singh, Kashyap Kumar Dubey and Bhanu P Singh. Partial purification and characterization of hide dehairing protease. J Pharmacogn Phytochem 2018;7(1S):2608-2611.

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