Author(s):

Lipne Vaibhav T, Kadu Tanvi P, Kale Sonam S, Toshy Agrawal and AS Kotasthane

Abstract: Bacterial leaf blight is a severe rice disease throughout the world that is controlled primarily through use of resistant cultivars. Marker assisted selection (MAS) is essential for improvement of resistance in breeding programs because of they are co-dominant, based on PCR amplification, represent single loci and detect high levels of polymorphism. As on date, 30 major genes have been reported to confer resistance against <em>Xanthomonas oryzae pv oryzae</em> (Xoo), which included 21 dominant and 9 recessive R genes. In the present investigation the behavior of R genes in population indicated that faster the response rate is more likely a resistant phenotype is to arise. The recessive R genes i.e <em>xa5 </em>(chromosome 5) which specifically confers resistance to BLB of rice only in recessive homozygous condition. The PCR based screening of selected 40 F₄individuals, each plants selected which derived from cross between IRBB5xIR64.The F₄ progeny were tested and inoculated artificially by <em>Xanthomonas pv. Oryzae pv oryzae </em>(Dhamtari) isolate<em>.</em> In this study microsatellite and sequence-tagged site(STS)were most practical markers used for marker assisted selection of the targeted BLB resistant genes <em>xa5</em>.Tagged rice line #1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 22, 24, 25, 26, 27, 28, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 derived from IRBB5xIR64 were resistant phenotypically and also show co-segregation with linked SSR marker RM610. These selected lines will further be phenotyped under field condition for resistance to BLB, further purification and for the restoration of the background.

Pages: 3024-3028  |  1266 Views  376 Downloads

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