Journal of Pharmacognosy and Phytochemistry
Vol. 7, Special Issue 1 (2018)
Identification of some French bean (<em>Phaseolus vulgaris</em> L.) genotypes through SDS-PAGE analysis of seed storage protein
Author(s):
PG Devhade, AK Basu and Nation Chamling
Abstract:
The present investigation was done to discriminate eight genotypes of French bean based on the nature of storage protein present in the seeds. Storage protein of harvest fresh seeds of eight genotypes viz., Sonali, Selection 9, Deepali, Abhay, Victoria, Arka Suvidha, Arka Anoop and Falguni were analysed through SDS-PAGE and the banding pattern for individual genotypes were utilized to discriminate one from the others. Total number of 11 protein bands could be identified with Rm values ranging from 0.11 to 0.97. All the 11 bands could be noticed for three genotypes only <em>viz</em>., Sonali, Arka Anoop and Falguni, 10 bands could be recognised for both Abhay and Arka Suvidha with absence of band number 8 and 7 respectively. While 3 bands each were absent for Selection 9 and Victoria differing in position of two bands, and Deepali was the single genotype for which 4 bands were found to be absent. Grouping of the genotypes initially into two as well as its sub-grouping indicate the relationship among themselves.
Pages: 3037-3039 | 1417 Views 366 Downloads
PG Devhade, AK Basu and Nation Chamling. Identification of some French bean (<em>Phaseolus vulgaris</em> L.) genotypes through SDS-PAGE analysis of seed storage protein. J Pharmacogn Phytochem 2018;7(1S):3037-3039.