Author(s):

Nagesha BV, Ugraiah Amilineni

Abstract: Camptothecine (CPT), monoterpene indole alksloid, is a potent inhibitor of eukaryotic DNA topoisomerase-I. CPT and its derivatives are being used in different treatments of several cancers. We have proved that biotechnological potential of <em>Ophiorrhiza mungos</em> for mass multiplication from <em>in vitro</em> grown plants an efficient protocol for the regeneration of endangered <em>O. mungos </em>plants using nodal explants. The nodal explants were inoculated on Murashige and Skoog (MS) medium fortified with different concentrations of plant growth regulator N⁶-benzyladenine (BA). The results revealed that the maximum number of shoots (20.06) with 100 % regeneration frequency from nodal explants obtained in MS medium fortified with 2mg/l BA within eight weeks. The present findings indicate that <em>O. mungos</em> respond favourably for <em>in vitro</em> propagation and these <em>in vitro</em> regenerated flowering plants of <em>O. mungos</em> can be used for over expression of key genes involved in regulating terpenoid indole alkaloid (CPT) biosynthesis.

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