Author(s):

Ranju Kumari, AK Singh and VK Sharma

Abstract: Seed is the basic and most critical input for sustainable agriculture. The quality seed is determined by its genetic purity in which the main source of genetic contamination is the self-pollination of the female parent due to incomplete removal of the tassel. Traditionally, the approach adopted for the genetic purity certification in maize is not accurate and fast method. Thus, the present investigation was carried out to test the genetic purity of single cross by using isozyme as a marker. The horizontal starch gel electrophoresis was used to study the isozyme patterns in eight inbred lines namely, CML 142, CML 144, CML 150, CML 178, CML 186, CM 300, CM 400, CM 600 and some selected fifteen single cross hybrids having high heterosis to distinguish single cross hybrids from their parental lines. The parental lines were obtained from AICRP on Maize, Dholi Centre and hybrids were generated by crossing these inbreds in all possible combinations excluding reciprocal. These single cross hybrids and their parental lines were evaluated by isozymatic pattern of peroxidase, catalase, amylase and esterase. Amongest these isozyme systems, banding pattern produced by peroxidase, catalase, and esterase isozyme systems made it possible to distinguish single crossed hybrids from their parental lines. Morever, this technique was fast, precise and without environmental effects.

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