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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Special Issue 5 (2018)

<em>In Vitro </em>callus induction and plant regeneration in basmati rice (<em>Oryza Sativa</em> L.) varieties

Author(s):

Shilpy Singh, Arun Kumar, Vivek Rana, Ashwini Kumar and V Rakesh Sharma

Abstract:
Rice is the most important staple food for a large part of the world’s population. Its production needs increase to meet the predicted demands of increasing population. Biotechnology provides a novel approach for rice improvement through transformation technique. Studies related to the <em>In-vitro </em>culture of plant require a basic protocol for callus induction and subsequent plant regeneration. Efficient callus induction and plant regeneration are pre-requisite for transformation. In the present study, an efficient callus induction and regeneration system was established for basmati rice varieties viz. Basmati-370, Pusa Sugandha-4, Vallabh Basmati-22 and Taroari. A number of factors such as media composition, different concentrations and combinations of hormones were studied. MS media supplemented with 2.0 mg/l of 2, 4-D produced the most desired calli. The callus induction frequency was found to be highest for Pusa Sugandha-4 while Basmati-370 showed the highest regeneration frequency on MS medium supplemented with 2.0 mg/l BAP and 1mg/l of NAA.

Pages: 65-69  |  1723 Views  382 Downloads

How to cite this article:
Shilpy Singh, Arun Kumar, Vivek Rana, Ashwini Kumar and V Rakesh Sharma. <em>In Vitro </em>callus induction and plant regeneration in basmati rice (<em>Oryza Sativa</em> L.) varieties. J Pharmacogn Phytochem 2018;7(5S):65-69.

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