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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Special Issue 5 (2018)

Isolation of probiotic <em>Lactobacillus SP</em> from raw cow milk and its usage as plant growth promoting bacteria

Author(s):

N Murgalatha Nallayan and Kanchana Devi

Abstract:
Chilli plant leaf spot is caused by bacteria belonging to several species in the genus <em>Xanthomonas </em>especialy <em>Xanthomonas campestris.</em> Infected chilli plant were collected from local area of Coimbatore and botanical name was confirmed as <em>Capsicum annum</em> and were processed for isolation of <em>Xanthomonas.</em> Among the processed samples colonies with typical characteristics namely smooth, round (1.5cm-2cm) with entire margins were picked and were subjected to morphological and biochemical characteristics. Isolates are reacted negatively to gram staining under a light microscope. <em>Xanthomonas campestris</em> were able to utilize citrate, gelatin is liquefied, indole is produced, catalase is positive, nitrate is reduced, starch and casein is hydrolysed, oxidase is positive. <em>Xanthomonas campestris</em> can able to grow on nutrient agar with 5% glucose. <em>Lactobacillus</em> sp is a probiotics bacteria found mainly associated with milk products. Raw cow milk sample were collected from the local area of Coimbatore and processed for isolation of <em>Lactobacillus.</em> Among the processed samples colonies with typical characteristics pure, white, small (2-3mm diameter) with entire margins were picked and were subjected to morphological and biochemical characteristics. The isolates were identified as <em>Lactobacillus acidophilus</em> and <em>Lactobacillus sporogenes</em>. The isolates reacted positively to gram staining under a light microscope. <em>Lactobacillus </em>sp not able to reduce nitrates, gelatin is not liquefied, indole is not produced, acidic and non acidic end products are not produced and are catalase negative. The cultures supernatant obtained from the bacteriocin producer strain were tested for the antibacterial activity against isolates of <em>Xanthomonas campestris</em> by agar well diffusion assay. Inhibitory zones shows that the strain <em>Lactobacillus acidophilus</em> show strong antimicrobial activity. For the <em>in vitro</em> efficacy of LAB as biocontrol and Plant Growth Promoting Bacteria, chilli seeds from two varieties (CO1, K2) were treated with LAB strain and treated seeds showed inhibitory activity against <em>Xanthomona campestris </em>strain. They can resist the bacterial infection and showed good percentage of increase in shoot length and root length in the seeds treated with <em>Lactobacillus acidophilus</em>. The use of chemicals and bacteriocides in agriculture as well as the environmental pollution would be avoided by LAB as a promising PGPB and Biocontrol agent.

Pages: 103-107  |  1750 Views  473 Downloads

How to cite this article:
N Murgalatha Nallayan and Kanchana Devi. Isolation of probiotic <em>Lactobacillus SP</em> from raw cow milk and its usage as plant growth promoting bacteria. J Pharmacogn Phytochem 2018;7(5S):103-107.

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