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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 7, Special Issue 5 (2018)

Phenotypic and genotypic characterization of <em>Salmonella</em> isolated from beef in northern India

Author(s):

Javed Ahamad Khan, Ram Swaroop Rathore, Hussein Hasan Abulreesh, Faizan Abul Qais and Iqbal Ahmad

Abstract:
Livestock industries are growing tremendously along with the human population. India has rapidly increased its beef exports. The maintenance of quality of food products derived from these animals is on high consideration. In this investigation the prevalence of <em>Salmonella</em> species was performed through phenotypic and genotypic characterization in beef samples (n=100) collected from butcher shops locally in Bareilly. A total of 8 isolates (4.0%, n = 8) were identified via. phenotypic assay (H<sub>2</sub>S production on TSI, Lysine decarboxylase assay, acid production from lactose and sucrose, IMViC assay). The genotypic characterization was performed using Polymerase Chain Reaction (PCR) assay targeting <em>inv</em>A gene (389 bp). Furthermore, antibacterial resistance profile of all <em>Salmonella </em>isolates (n=8) was determined against seven antibiotics (ampicillin, cephalexin, ciprofloxacin, nalidixic acid, gentamicin, tetracycline and streptomycin. The antibiotic susceptibility profiles confirmed drug resistance among 50% of <em>Salmonella </em>isolates. It was observed that 37.5% of <em>Salmonella</em> isolates were resistant to the ampicillin and ciprofloxacin whereas a slight lower resistance of 25.0% against ciprofloxacin and tetracycline was observed. No resistance was observed against nalidixic acid, gentamycin and streptomycin. Overall resistance to multiple drugs (≥ 3) was recorded in 25.0% isolated strains.

Pages: 133-136  |  1172 Views  201 Downloads

How to cite this article:
Javed Ahamad Khan, Ram Swaroop Rathore, Hussein Hasan Abulreesh, Faizan Abul Qais and Iqbal Ahmad. Phenotypic and genotypic characterization of <em>Salmonella</em> isolated from beef in northern India. J Pharmacogn Phytochem 2018;7(5S):133-136.

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