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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Special Issue 1 (2019)

Genetic diversity of bread wheat genotypes (<em>Triticum aestivum </em>L.) revealed by microsatellite SSR markers for leaf and stripe rust resistance

Author(s):

Pooja, Veena Chawla and Prince

Abstract:
The present investigation was conducted to evaluate 40 diverse genotypes of bread wheat and to characterize these genotypes using Simple Sequence Repeat (SSR) markers. A total of 27 SSR primers were used in study. Three SSRs (<em>Xgwm 582, LrK10-1AS </em>and <em>gwm344) </em>did not show amplification of genomic DNA from different wheat genotypes. Only 24 SSRs (88.8%) showed amplification, of which 11 SSRs (40.7%) produced polymorphic bands while 13 SSRs produced monomorphic bands (44.4%). Size of amplified product in different genotypes ranged from 150-400 bp, a total of 44 alleles were detected with 1.83 as the average number of alleles detected.

Pages: 86-93  |  1685 Views  578 Downloads

How to cite this article:
Pooja, Veena Chawla and Prince. Genetic diversity of bread wheat genotypes (<em>Triticum aestivum </em>L.) revealed by microsatellite SSR markers for leaf and stripe rust resistance. J Pharmacogn Phytochem 2019;8(1S):86-93.

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