Author(s):

Reetika Panwar, Chandel S and Rani S

Abstract: Rose (<em>Rosa</em> sp.) belongs to family <em>Rosaceae</em>. Black spot, <em>Diplocarpon rosae,</em> is a fungal disease specific to roses. It is caused by a common fungus that thrives in wet, warm, and humid conditions. It is dominant and occurs worldwide and is the most severe disease of rose in almost all species and cultivars of Rose. The fungus infects leaves in the spring and reduces the plant’s vigor throughout the growing season. In the present research, Cultural studies of <em>Marssonina rosae</em> were carried under in vitro conditions in order to find out the best liquid and solid medium for the vegetative growth and sporulation of the test fungus. Out of five solid media maximum radial growth was recorded on Malt Extract Agar (76.56mm) with sporulation count of 55.02 x 104 and out of five liquid media maximum dry mycelial weight (292.20mg) and sporulation of 24.46 x 104 spores/ml was observed on Richard’s medium.

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