Author(s):

Manish Kapoor and Jyoti Rani

Abstract: The present study was framed to evaluate bioactive phytochemical, antimicrobial and antioxidant properties of the sequentially extracts prepared from leaves of <em>Catharanthus roseus </em>in five different solvents (Petroleum ether, Chloroform, ethanol, methanol and aqueous). Antioxidant potential of the extracts was evaluated by both enzymatic assays (Superoxide dismutase, Catalase, Glutathione peroxidase) and non-enzymatic assays (DPPH, ABTS). Lowest IC₅₀ values were shown by methanolic extracts followed by ethanol and aqueous extracts. The antioxidant activity of enzymes <em>viz</em>. catalase, glutathione peroxidase and superoxide dismutase were found to be satisfactory. The antioxidant activity of the extracts exhibited positive correlation with the total phenolic<strong> </strong>and flavonoid content of the extracts. The chemical composition of methanolic extract was also analysed by GC-MS. Seven phytochemical were predominantly identified in the methanolic extract by GCMS.

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