Author(s):

Kaur Rupinder, Kaur Harmanjot and Rani Shalu

Abstract: The present work was carried out to isolate industrially important enzyme producing strain. The soil samples from different areas <em>viz</em>. Rhizospheric, Nonrhizospheric and Landfill areas were collected, appropriate dilution of different soil samples were plated &amp; CFU was calculated. The microbial diversity was also checked &amp; mix culture gram (+)ve &amp; gram (-ve) was observed. These isolates were screen for the presence of various enzymes mannanase, xylanase, laccase, protease &amp; amylase, of the various isolates mannanase producing strains was selected for further study. The sample were plated on a medium with LBG to isolate mannanase producing colony of the RN 11 isolates, strain showing maximum zone of clearance was further selected. Selected strain was subjected to morphological &amp; biochemical characterization which showed the <em>Bacillus species</em>. On the flask level mannanase activity was checked &amp; after 4 hrs enzyme activity 25 IU was observed. The medium was characterized in terms of pH &amp; temperature. Thereafter the strain was used for the bio bleaching of kraft pulp &amp; it reduced the kappa number by 14.86%.

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