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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Special Issue 2 (2019)

Validation of reported markers for seed dormancy and pre-harvest sprouting resistance in rice (<em>Oryza sativa.</em> L)

Author(s):

G Bharathi, B Krishna Veni, CN Neeraja, M Lal Ahamed and K Jaya Lalitha

Abstract:
DNA markers have enormous potential to improve efficiency and precision of conventional plant breeding <em>via</em> marker assisted selection. To test the usefulness of microsatellite markers associated with pre-harvest sprouting resistance QTLs in rice, 32 diverse rice genotypes and two F<sub>2</sub> populations were used. A total of 24 reported SSR markers were used to reveal polymorphism between dormant and non-dormant rice genotypes. Among the 24 tested SSR primers five markers <em>viz.,</em> RM252, RM17, RM564, RM480 and RM346 showed polymorphism for pre-harvest sprouting resistance among 32 genotypes. The phenotyping results revealed that MTU 1075, MTU 3626, MTU 5293, MTU 1010, BPT 2658, BPT 2741, BPT 2411, RGL 1414 and BPT 3291 possess moderate dormancy for 12 DAH while MTU 1001 exhibited strong dormancy. The phenotypic germination patterns of 32 genotypes at different intervals were compared with the genotyping results using Map Disto method of analysis. The test of goodness of fit χ<sup>2</sup> test was conducted for phenotypic germination patterns of 32 genotypes and genotypic data developed by five polymorphic markers and the results revealed that two markers <em>i.e.,</em> RM252 and RM17 exhibited significance. Among the 6 markers studied, the primers RM21 and RM252 were able to show distinct polymorphism between dormant and non-dormant genotypes among the individuals selected from BPT 5204/ MTU 1001 F<sub>2 </sub>population while RM480 and RM235 showed a good characteristic polymorphism among the selected individuals of BPT 2231/MTU 1001 F<sub>2</sub> population.

Pages: 103-108  |  1260 Views  211 Downloads

How to cite this article:
G Bharathi, B Krishna Veni, CN Neeraja, M Lal Ahamed and K Jaya Lalitha. Validation of reported markers for seed dormancy and pre-harvest sprouting resistance in rice (<em>Oryza sativa.</em> L). J Pharmacogn Phytochem 2019;8(2S):103-108.

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